Recently micro vascular anastomoses have been developed for wide employment in clinical appliance. However, in end-to end anastomoses of arteries less than 1mm in diameter, the vascular occlusions have been often occurred due to suture material, suture method and aneu-rysm, thromboses and infection at the site of repair.
To obtain the more successful microvascular method and research the histopathology of the vessel wall after repair, We did the experimental comparison of the different methods of microvascular anastomoses in rat femoral artery less than 1mm in diameter.
The experiment was done in 3 different groups:
In group 1: 6-8 sutures was done.
In group 2 6-8 sutures and application of autogenous fascia cuff to the anastomotic site was done.
In group 3: 4 sutures and application of autogenous fascia cuff to the anastomotic site was done.
The results are summarized as follows:
1) Patency rate was 90% in group 1, 100% in group 2,3 and thromboses was 10% in group 1, absent in group 2, 3.
2) Aneurysm formation was 30% in group 1, 10%/ in group 2, 3. 3) Infection was absent in all 3 groups.
4) The histopathology of the microvascular anastomotic site revealed intimal loss, medial necrosis, elastic lamella loss and subintimal hyperplasia in all groups.
5) The histopathologic comparison of the outer surface of the vessel wall in each groups revealed as following:
(1) group I : neovascularization and more focal aneurysm to the anastomotic site.
(2) group 2,3: neovascularization, less focal aneurysm and autogenous fascia was well attached to the vessel wall.
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